Effect of Ephedra Herb on Erlotinib Resistance in c-Met-Overexpressing Non-Small-Cell Lung Cancer Cell Line, H1993, through Promotion of Endocytosis and Degradation of c-Met

Figure 7

Expression of c-Met and pMet in H1993 cells after treatment with bafilomycin A1 and EHE. H1993 cells were incubated for 1 h at 37°C in RPMI-1640 medium or RPMI-1640 medium containing 50 µg/ml or 100 µg/ml bafilomycin A1 and 50 µg/ml EHE. The expression levels of c-Met, pMet, and GAPDH were analyzed by western blotting (a). The density of each band was analyzed with an ImageQuant Las 4000 mini system without saturating the signal. The value is expressed as follows: ((the density the c-Met or pMet band in cells treated with the test drug/the density of the GAPDH band in cells treated with the test drug)/(the density the c-Met or pMet band in control cells/the density of the GAPDH band in control cells)) ((b) and (c)). Each error bar represents the average (n = 3) ± S.D. Data were analyzed by 1-way ANOVA (C : DF = 2, F(2, 9) = 3.831, and ). Significant differences were determined by Dunnett’s test; C: vs. 50 µg/ml EHE.

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