Effect of Ephedra Herb on Erlotinib Resistance in c-Met-Overexpressing Non-Small-Cell Lung Cancer Cell Line, H1993, through Promotion of Endocytosis and Degradation of c-Met

Figure 8

Expression of HGF-c-Met downstream signaling molecules in H1993 cells after treatment with HGF and EHE. (a) H1993 cells were incubated for 4 h at 37°C in 4 ml RPMI-1640 containing 50 ng/ml HGF and 0–200 µg/ml EHE. The expression levels of pMet, c-Met, and GAPDH were analyzed by western blotting. (b) H1993 cells were incubated for 0, 1, 2, 4, and 6 h at 37°C in 4 ml RPMI-1640 containing 50 ng/ml HGF or RPMI-1640 containing 50 ng/ml HGF and 50 µg/ml EHE. The expression levels of c-Met downstream signaling molecules and GAPDH were analyzed by western blotting.

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