Gukang Capsule Promotes Fracture Healing by Activating BMP/SMAD and Wnt/β-Catenin Signaling Pathways

Figure 4

Effects of GKC on Wnt/β-catenin signaling during the osteogenic differentiation of SaOS-2 cells. (a) RT-qPCR was used to measure mRNA expression levels of the Wnt/β-catenin signaling pathway-related key gene β-catenin, and western blot analysis was used to measure the protein levels of β-catenin, GSK3β, and phosphorylated GSK3β after 24 h of GKC treatment at different concentrations (n = 3). (b) Representative western blots showing BMP2 and RUNX2 protein expression of SaOS-2 cells, with or without DKK1 pretreatment (5 h 0.5 μg/ml), treated with or without 200 mg/l GKC for 24 h (n = 3). (c) ALP staining (7 days) and Alizarin Red staining (14 days) of SaOS-2 cells after DKK1 treatment (n = 3). and , relative to the control group. , relative to the GKC treatment groups.

(a)

(b)

(c)