Rhein Suppresses Neuroinflammation via Multiple Signaling Pathways in LPS-Stimulated BV2 Microglia Cells

Figure 6

Effects of Rhein on TLR4/NF-κB pathway components in LPS-stimulated BV2 cells. The cells were untreated (control) or pretreated with Rhein/DMSO for 30 min, followed by LPS stimulation (1 μg/mL) for 48 h. WB analysis was carried out to evaluate the protein levels of TLR4, p-IκBα, and NF-κB p65. GAPDH as internal control for total protein and cytoplasmic protein; Lamin B as internal control for nucleoprotein. Data represent the mean ± SEM of three independent experiments. , , and .

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