Integrated Network and Experimental Pharmacology for Deciphering the Medicinal Substances and Multiple Mechanisms of Duhuo Jisheng Decoction in Osteoarthritis Therapy

Figure 5

Effects of DHJS on cell viability and IL-1β-induced expression of TNFα, IL-6, MMPs, and ADAMTs-5 in rat chondrocytes. Identification of rat primary chondrocytes (a). Rat chondrocytes were shaped like spindles with a protuberance, in which proteoglycans were stained purple by toluidine blue. Effects of DHJS on cell viability of chondrocytes (b). Effects of DHJS on cell viability of IL-1β-induced chondrocytes (c). Effects of DHJS on IL-1β-induced expression of TNFα (d), IL-6 (e), MMP-1 (f), MMP-3 (g), MMP-9 (h), and ADAMTs-5 (i) in rat chondrocytes. Data are expressed as mean ± SD (n = 3). , , and , compared between the marked groups.

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