Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model

Figure 2

Effect of hirudin on renal interstitial ECM accumulation and activation of myofibroblast in UUO rats. (a) Immunohistochemical staining of FN and α-SMA (magnification ×400). (b) Western blot analysis of FN, collagen III, and α-SMA; GAPDH was used as the loading control. (c–e) Protein levels of FN, collagen III, and α-SMA in each group. (f, g) Average optical density of FN and α-SMA. Data are expressed as mean ± SD. For comparison among the UUO group and the control group, indicates . For comparison among the hirudin-treated groups and UUO group, indicates and indicates .

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