Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model

Figure 5

Effect of hirudin on protein expression of IL-6, TNF-α, and NF-κB signaling in UUO rats. (a, b) Western blot analysis of IL-6, TNF-α, p-iκBα, iκBα, p-P65, and P65; GAPDH was used as the loading control. (c–h) Protein levels of IL-6, TNF-α, p-iκBα, iκBα, p-P65, and P65 in each group. Data are expressed as mean ± SD. For comparison among the UUO group and the control group, indicates . For comparison among the hirudin-treated groups and UUO group, indicates and indicates .

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