Sikyungbanha-Tang Suppressing Acute Lung Injury in Mice Is Related to the Activation of Nrf2 and TNFAIP3

Figure 1

Cytotoxicity by SKBHT. (a) For measuring intracellular ROS induced by SKBHT, RAW 264.7 cells were treated with PBS (Con), LPS (100 ng/ml), or two different amounts of SKBHT (100 g/ml and 200 g/ml in PBS) for 16 h Cells were stained with carboxy-H2DCFDA, a ROS indicator, and analyzed by FACS. Cells producing intracellular ROS were shown in percentile at the right side of each panel. A similar experiment was repeated three times, and we obtained similar results. (b) Cytotoxicity due to interference of cellular metabolism by SKBHT was determined by MTT assay. RAW 264.7 cells were treated with increasing amounts of SKBHT for 16 h orcid:0000-0002-5417-1732 Metabolically active cells were determined by measuring the formazan product with a spectrophotometer. Data represent the mean ± SEM of triplicate samples. A similar experiment was repeated twice, and we obtained similar results. No statistical differences between groups were found (ANOVA).

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