The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells

Figure 5

Effect of SMI on TH phosphorylation in bovine adrenal medullary cells. Phosphorylation at the Ser19, Ser31, and Ser40 sites of TH was determined and expressed as % of control TH band. (a) Effect of SMI on TH phosphorylation at Ser19, Ser31, and Ser40. (b) Effect of SMI on the ratio of the density of TH Ser19 phosphorylation induced by 300 μM ACh. (c) Effect of SMI on the ratio of the density of TH Ser31 phosphorylation induced by 300 μM ACh. (d) Effect of SMI on the ratio of the density of TH Ser40 phosphorylation induced by 300 μM ACh. ((e), (g)) Effect of SMI and CaM kinase II on phosphorylation of TH Ser19 induced by 300 μM ACh. ((f), (h)) Effect of SMI and PKA on phosphorylation of TH Ser40 induced by 300 μM ACh. Data are expressed as mean ± SEM based on three independent experiments each performed in triplicates. and compared with ACh alone. and compared with control.

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