Xanthomicrol Exerts Antiangiogenic and Antitumor Effects in a Mouse Melanoma (B16F10) Allograft Model

Figure 6

Xanthomicrol inhibited HIF-1α/VEGF axis and release of VEGF in vivo. After 21 consecutive days of treatment, animals were sacrificed, and tumor tissues were excised and used for quantitative RT-PCR analysis of HIF-1α (a) and VEGF (b) mRNA expression. HPRT1 expression was used as housekeeping gene. Blood samples were also collected by cardiac puncture and levels of serum VEGF were determined using an ELISA kit (c). Data are represented as mean ± SEM (^### versus normal, nontumor-bearing mice; ^∗∗ and ^∗ vs. vehicle-treated mice).

(a)

(b)

(c)