Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts of Sesamum angustifolium (Oliv.) Engl. and Hibiscus articulatus on Rhabdomyosarcoma

Table 4

Propidium iodide assay to determine apoptosis induction effect of SAHA extracts and doxorubicin in RD and L20B cells after 48 hour treatment.

Extract	Concentration (μg/mL)	Viability (T/C) (%)
		RD cells	L20B cells
Doxorubicin	8	49.7 ± 5.7	57.7 ± 17.2
	2	68.2 ± 8.1	68.2 ± 14.7
Dichloromethane	200	55.1 ± 3.2	66.7 ± 14.4
	50	63.5 ± 12.3	60.0 ± 10.1
Acetone	200	60.9 ± 1.0	78.3 ± 33.6
	50	66.6 ± 8.6	60.5 ± 4.5
Methanol	200	48.4 ± 2.7	65.1 ± 10.8
	50	50.9 ± 7.8	55.6 ± 13.0
Aqueous	200	72.1 ± 3.2	85.9 ± 13.4
	50	93.0 ± 10.6	83.4 ± 10.0

The number of live negative control cells (0 μg/mL) at 48 h was used as 100% viability, so all cells were expressed as a percentage of them. Results are mean ± SD of two replicates (n = 2). indicates comparing treated RD cells to treated L20B cells of similar concentration.