HPLC-MS/MS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach

Table 3

Precision and accuracy of the eight probe drugs in rat plasma (n = 6).

Probe drug	Spiked conc. (ng/mL)	Intraday			Interday
		Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (RE, %)	Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (RE, %)
7-Hydroxycoumarin	25	22.0 ± 2.0	9.1	−12.0	24.6 ± 2.5	10.2	−1.6
	500	523.1 ± 33.4	6.4	4.6	517.0 ± 26.6	5.1	3.4
	5000	4344.9 ± 251.1	5.8	−13.1	4375.8 ± 218.6	5.0	−12.5
Omeprazole	10	10.0 ± 0.8	8.0	0.0	10.6 ± 0.8	7.5	6.0
	200	228.7 ± 8.9	3.9	14.4	222.6 ± 10.5	4.7	11.3
	2000	1720.9 ± 96.0	5.6	−14.0	1772.9 ± 104.2	6.0	−11.4
Dextromethorphan	2.5	2.3 ± 0.3	13.0	−8.0	2.5 ± 0.3	12.0	0.0
	50	52.7 ± 2.8	5.3	5.4	50.8 ± 2.6	5.1	1.6
	500	476.8 ± 41.3	8.7	−4.6	489.1 ± 31.1	6.4	−2.2
Bupropion	5	4.3 ± 0.2	4.7	−14.0	4.8 ± 0.2	4.2	−4.0
	100	98.6 ± 7.7	7.8	−1.4	107.7 ± 11.0	10.2	7.7
	1000	1063.5 ± 80.3	7.6	6.4	1052.9 ± 101.3	9.6	5.3
Phenacetin	5	4.5 ± 0.5	11.1	−10.0	5.0 ± 0.6	12.0	0.0
	100	98.3 ± 6.2	6.3	−1.7	101.1 ± 7.8	7.7	1.1
	1000	923.1 ± 49.8	5.4	−7.7	913.0 ± 53.6	5.9	−8.7
Dapsone	2.5	2.3 ± 0.1	4.3	−8.0	2.6 ± 0.3	11.5	4.0
	50	55.4 ± 2.9	5.2	10.8	52.9 ± 3.9	7.4	5.8
	500	492.7 ± 36.8	7.5	−1.5	482.6 ± 31.8	6.6	−2.5
Tolbutamide	15	14.5 ± 0.6	4.1	−3.3	15.4 ± 0.6	3.9	2.7
	300	317.6 ± 12.9	4.1	5.9	311.0 ± 12.8	4.1	3.7
	3000	3034.3 ± 178.8	5.9	1.1	3029.8 ± 140.4	4.6	1.0
Amodiaquine	5	5.5 ± 0.3	5.5	10.0	4.9 ± 0.3	6.1	−2
	100	113.1 ± 3.7	3.3	13.1	112.1 ± 4.7	4.2	12.1
	1000	860.4 ± 41.8	4.9	−14.0	913.2 ± 60.9	6.7	−8.7