HPLC-MS/MS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach

Table 4

Extraction recoveries and matrix effects of the eight probe drugs in rat plasma (n = 6).

Probe drug	Spiked conc. (ng/mL)	Extraction recovery (%)	RSD (%)	Matrix effect (%)	RSD (%)
7-Hydroxycoumarin	25	102.6 ± 4.6	4.5	81.5 ± 1.7	2.1
	500	106.4 ± 7.8	7.3	93.6 ± 5.1	5.4
	5000	109.4 ± 5.0	4.6	95.4 ± 1.0	1.0
Omeprazole	10	90.1 ± 6.0	6.7	95.9 ± 6.4	6.7
	200	103.1 ± 6.6	6.4	96.3 ± 4.4	4.6
	2000	99.6 ± 2.7	2.7	99.4 ± 1.2	1.2
Dextromethorphan	2.5	109.3 ± 9.4	8.6	105.6 ± 6.7	6.3
	50	106.9 ± 4.5	4.2	94.7 ± 2.5	2.6
	500	99.3 ± 3.1	3.1	100.4 ± 1.8	1.8
Bupropion	5	85.0 ± 9.9	11.6	93.3 ± 7.1	7.6
	100	85.2 ± 3.1	3.6	90.6 ± 1.3	1.4
	1000	87.8 ± 7.8	8.9	100.0 ± 0.8	0.8
Phenacetin	5	94.7 ± 8.3	8.8	92.9 ± 4.6	5.0
	100	106.4 ± 9.1	8.6	92.5 ± 5.0	5.4
	1000	106.9 ± 5.9	5.5	96.4 ± 1.5	1.6
Dapsone	2.5	98.7 ± 8.5	8.6	90.3 ± 5.7	6.3
	50	103.4 ± 4.9	4.7	89.3 ± 1.8	2.0
	500	103.9 ± 3.8	3.7	97.7 ± 1.6	1.6
Tolbutamide	15	86.5 ± 6.5	7.5	87.3 ± 1.6	1.8
	300	85.9 ± 3.2	3.7	90.2 ± 2.5	2.8
	3000	91.9 ± 2.7	2.9	93.9 ± 0.8	0.9
Amodiaquine	5	91.5 ± 4.5	4.9	85.5 ± 4.9	5.7
	100	93.8 ± 9.2	9.8	100.0 ± 9.9	9.9
	1000	95.4 ± 3.9	4.1	102.2 ± 3.1	3.0