HPLC-MS/MS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach

Table 5

Stability results of the eight probe drugs in rat plasma (n = 6).

Probe drug	Spiked conc. (ng/mL)	4 h at room temperature		3 freeze-thaw cycles		12 h in autosampler		−70°C for 14 days
		Measured conc. (ng/mL)	RSD (%)	Measured conc. (ng/mL)	RSD (%)	Measured conc. (ng/mL)	RSD (%)	Measured conc. (ng/mL)	RSD (%)
7-Hydroxycoumarin	25	25.4 ± 1.2	4.7	24.6 ± 0.6	2.4	27.1 ± 2.0	7.4	25.7 ± 1.4	5.4
	500	558.8 ± 11.9	2.1	498.5 ± 33.8	6.8	529.9 ± 7.2	1.4	537.4 ± 27.5	5.1
	5000	5063.6 ± 247.0	4.9	4263.2 ± 145.5	3.4	4472.6 ± 505.3	11.3	4358.0 ± 117.4	2.7
Omeprazole	10	11.1 ± 0.5	4.5	11.2 ± 0.3	2.7	10.5 ± 1.0	9.5	11.2 ± 0.1	0.9
	200	228.8 ± 4.5	2.0	228.9 ± 9.1	4.0	223.6 ± 8.3	3.7	228.8 ± 2.6	1.1
	2000	2061.6 ± 77.2	3.7	1730.0 ± 57.4	3.3	1842.8 ± 100.4	5.4	1844.5 ± 24.5	1.3
Dextromethorphan	2.5	2.5 ± 0.2	8.0	2.2 ± 0.1	4.5	2.6 ± 0.3	9.8	2.1 ± 0.1	4.8
	50	55.9 ± 0.8	1.4	51.3 ± 4.0	7.8	52.2 ± 3.7	7.2	56.0 ± 1.4	2.5
	500	560.0 ± 18.9	3.4	469.8 ± 17.5	3.7	503.9 ± 51.6	10.2	493.9 ± 14.4	2.9
Bupropion	5	4.9 ± 0.6	12.2	4.4 ± 0.5	11.4	4.2 ± 0.2	4.5	5.4 ± 0.2	3.7
	100	114.0 ± 3.5	3.1	112.0 ± 10.7	9.6	98.3 ± 12.6	12.9	104.1 ± 13.9	13.4
	1000	1135.8 ± 50.7	4.5	1008.7 ± 144.1	14.3	959.1 ± 44.4	4.6	1128.5 ± 73.4	6.5
Phenacetin	5	5.1 ± 0.2	3.9	4.4 ± 0.3	6.8	5.0 ± 0.3	5.5	4.9 ± 0.4	8.2
	100	113.1 ± 3.1	2.7	102.3 ± 9.8	9.6	100.1 ± 4.7	4.7	111.2 ± 5.7	5.1
	1000	1021.4 ± 49.1	4.8	867.7 ± 31.3	3.6	850.6 ± 35.0	4.1	873.7 ± 32.2	3.7
Dapsone	2.5	2.5 ± 0.2	8.0	2.6 ± 0.2	7.7	2.6 ± 0.4	13.5	2.5 ± 0.2	8.0
	50	56.0 ± 1.8	3.2	55.7 ± 3.7	6.6	52.1 ± 2.4	4.5	56.1 ± 1.4	2.5
	500	559.7 ± 33.1	5.9	487.6 ± 15.8	3.2	470.8 ± 64.1	13.6	528.2 ± 21.0	4.0
Tolbutamide	15	14.7 ± 0.7	4.8	13.0 ± 0.5	3.8	15.3 ± 0.2	1.6	14.8 ± 0.3	2.0
	300	332.9 ± 10.6	3.2	292.4 ± 19.4	6.6	318.6 ± 16.9	5.3	316.2 ± 10.9	3.4
	3000	3397.6 ± 217.0	6.4	2725.3 ± 114.1	4.2	3425.5 ± 170.8	5.0	2864.3 ± 40.3	1.4
Amodiaquine	5	4.5 ± 0.4	8.9	5.0 ± 0.7	14.0	5.4 ± 0.3	5.1	5.4 ± 0.2	3.7
	100	112.1 ± 5.1	4.5	91.8 ± 5.4	5.9	106.3 ± 2.0	1.9	86.6 ± 6.8	7.9
	1000	926.3 ± 3.6	0.4	861.1 ± 38.7	4.5	917.9 ± 53.7	5.9	851.3 ± 6.6	0.8