Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity

Figure 6

Effect of paeoniflorin, paeonol, and cinnamaldehyde on COX2 activity and against induced dysmenorrhea. (a) The major components in GZFLc were paeoniflorin, paeonol, and cinnamaldehyde (refer to Section 2). Their effect against COX2 activity was determined using an enzyme-based assay. (b) Paeoniflorin, paeonol, and cinnamaldehyde were tested against induced dysmenorrhea at the following dosages: paeoniflorin at 5 and 10 mg/kg, paeonol at 5 and 10 mg/kg, and cinnamaldehyde at 1 and 2 mg/kg. The dosages were selected since they were close to the amounts in GZFLc at 300 mg/kg (refer to Section 2). GZFLc at 300 mg/kg and ibuprofen at 50 mg/kg were included as controls. Both writhing responses and the time of mice staying on the rotating bar were recorded. The experiment was performed twice independently.

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