Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity

Table 2

Inhibition of writhing responses by GZFLc in an oxytocin-induced dysmenorrhea model.

Group (n)	Oxytocin (i.p.)	Treatment (p.o.)	Writhing
Control (5)	−	Saline	N. S.
Mock (10)	+	Saline	14.7 ± 2.7
Ibuprofen (10)	+	Ibuprofen, 50 mg/kg	0.5 ± 0.7
GZFLc 150 (10)	+	GZFLc, 150 mg/kg	6.0 ± 1.9
GZFLc 300 (10)	+	GZFLc, 300 mg/kg	3.3 ± 1.3

ICR female mice were treated with estradiol for three consecutive days. The mice were randomly grouped into five groups. Thirty minutes prior to oxytocin injection, the mice were treated with normal saline, with ibuprofen, or with GZFLc at 150 and 300 mg/kg by oral gavage. To induce dysmenorrhea, mice were injected with oxytocin or with saline by i.p. route. The frequencies of pelvic twisting and rear limb stretching were recorded for 30 min and plotted as a measure of writhing responses (mean ± SD). The experiment was performed two times independently.