So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo

Figure 7

Effects of SOCG on the expression of HPA axis-related molecules in SH-SY5Y cells. (a) The viability of SH-SY5Y cells was determined by the MTT assay after they were cocultured with various concentrations of SOCG for 24  h. The cells were pretreated with SOCG for 1 h followed by incubation with corticosterone (100 μM) for 24 h for measuring the (b) BDNF, (c) GR, and (d) CRH mRNA expression levels. Total RNA was isolated and the levels of genes were measured by real-time PCR. The data are presented as the mean ± SEM. GAPDH was used for the normalization of the expression levels of the target genes in the real-time PCR analysis. ###, significant difference compared to the naive group. , , and , significant difference compared to the control group. CORT: corticosterone.

(a)

(b)

(c)

(d)