Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKT/GSK-3β/β-Catenin Pathway in Normal Human Epidermal Melanocytes

Figure 2

Effects of AS-IV on cell viability and melanogenesis in NHEMs. NHEMs were incubated with various concentrations (0, 1, 10, and 100 μM) of AS-IV for 48 h for (a) morphology (magnification: 40x) and (b) cell viability; NHEMs were incubated with various concentrations (0, 1, 10, and 100 μM) of AS-IV or 8-MOP (100 μM) for 24 h for (c) melanin synthesis detection and (d) tyrosinase activity. Results were expressed as mean ± SD (n = 3) based on the representative experiment of at least three replicates. and , versus untreated cells; , versus AS-IV-treated (100 μM) cells according to one-way ANOVA followed by Tukey’s multiple comparisons.

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