Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKT/GSK-3β/β-Catenin Pathway in Normal Human Epidermal Melanocytes

Figure 6

AKT/GSK-3β/β-catenin pathway was controlled by AhR activation in response to AS-IV in NHEMs. NHEMs were incubated with AS-IV (100 μM) for 24 h with or without pretreatment with AKT inhibitor IV (10 μM) or CH223191 (10 μM) for 3 h. Protein expressions of CYP1A1, p-AKT, AKT, p-GSK-3β, GSK-3β, MITF, and nuclei β-catenin were analyzed using (a) western blot and (b) quantitative analyses; (c) melanin synthesis detection. Results were expressed as mean ± SD (n = 3). and according to one-way ANOVA followed by Dunnett’s multiple comparisons.

(a)

(b)

(c)