Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation

Table 2

The effect of different concentrations of YNJ-medicated serum on the proliferation of INS-1 cells cultured under high glucose and high lipid (HG/HL) conditions (mean ± SD).

Group	n	Absorbance at 590 nm (A590)	95% confidence interval
			Lower limit	Upper limit
Control group	3	2.38 ± 0.17	2.17	2.60
HG/HL group	3	1.75 ± 0.32	1.53	1.96
HG/HL+5% YNJ-medicated serum group	3	2.11 ± 0.09^△	1.90	2.33
HG/HL+10% YNJ-medicated serum group	3	2.35 ± 0.16^△	2.14	2.57
HG/HL+20% YNJ-medicated serum group	3	2.09 ± 0.13^△	1.87	2.30

INS-1 cells in the logarithmic growth phase were divided into five groups: (1) control group (continue cultured with RPMI-1640 medium and all supplemented components); (2) HG/HL group (cultured with 25 mmol/L glucose, 0.5 mmol/L palmic acid, and 20% normal saline serum); (3) HG/HL+5% YNJ-medicated serum group (cultured with 25 mmol/L glucose, 0.5 mmol/L palmic acid, 5%YNJ-medicated serum, and 15% normal saline serum); (4) HG/HL+10% YNJ-medicated serum group (cultured with 25 mmol/L glucose, 0.5 mmol/L palmic acid, 10% YNJ-medicated serum, and 10% normal saline serum); and (5) HG/HL+20% YNJ-medicated serum group (cultured with 25 mmol/L glucose, 0.5 mmol/L palmic acid, 20%YNJ-medicated serum). MTT assay was used to detect the proliferation of INS-1 cells after 48 h of culture. Compared with the control group, ; compared with the HG/HL group, .