Jian-Pi-Yi-Shen Regulates EPO and Iron Recycling Protein Expressions in Anemic Rats with Chronic Kidney Disease: Accumulation of Hypoxia Inducible Factor-2α via ERK Signaling

Figure 6

Effects of JPYS on the serum hepcidin level, and the proteins expression of FPN and ferritin in rats. (a) The level of hepcidin in serum from different groups (n = 6). (b) Representative Western blot images of FPN and ferritin in the liver. (c) Liver proteins expression of FPN and ferritin relative to GAPDH. (d) Representative Western blot images of FPN and ferritin in the spleen. (e) Spleen proteins expression of FPN and ferritin relative to GAPDH. The results were presented as the means ± standard deviations (n = 6 or 3; , , compared with the sham group; , compared with the RA group; , compared with the LJPYS group). JPYS, Jian-Pi-Yi-Shen formula; FPN, ferroportin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RA, renal anemia; LJPYS, low dose Jian-Pi-Yi-Shen (1.5 g/kg/d); HJPYS, high dose Jian-Pi-Yi-Shen (6.0 g/kg/d).

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