Research Article
Arenobufagin Promoted Oxidative Stress-Associated Mitochondrial Pathway Apoptosis in A549 Non-Small-Cell Lung Cancer Cell Line
Figure 3
The effect of ARE on proapoptotic protein expression and MMP. A549 cells were treated with ARE (10–40 nM) for 24 h. (a) ARE-induced cleaved PARP, cleaved caspase-3, and caspase-9 expression. (b) ARE increased Bax expression but reduced Bcl-2 expression. (c) A549 cells are administered 24 hours after ARE administration; they were stained with JC-1. After that the mitochondrial membrane potential was observed under the fluorescence microscope. Scale bars: 100 μm. The results are expressed as mean ± SD (n = 3). versus the control group.
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