Plastrum Testudinis Extracts Promote NSC Differentiation into Dopaminergic Neuron by Regulating the Interaction of TET1 and FoxA2

Figure 6

siRNA silencing of Tet1 and Foxa2 gene. (a) siRNA silencing of Tet1 gene. The expression of Tet1 was assessed by qRT-PCR, and the efficiency of silencing was evaluated. (b) siRNA silencing of Foxa2 gene. The expression of Foxa2 was assessed by qRT-PCR. (c) Silencing of Tet1 was done with interference fragment siTet1_001, and the expression of mRNA level of Tet1, Th, and Foxa2 was assessed. (d) Expression of mRNA level of Th by qRT-PCR analysis. siRNA silencing of Tet1 was done in neural stem cells for 24 h and cells were cultured with PTE (30 μg/ml) for 24 h. (e) Silencing of Foxa2 was done with interference fragment siFoxa2_002, and the expression of mRNA level of Tet1, Th, and Foxa2 was assessed. (f) Expression of mRNA level of Th by qRT-PCR analysis. At the differentiation stage, siRNA silencing of Foxa2 were done in neural stem cells for 24 h and cells were cultured with PTE (30 μg/ml) for 24 h. compared with control, NC: NControl, ns: not significant; : values. The description of the statistical analyses is provided in Supplementary Tables S7–S12.

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