Sesamin Promotes Neurite Outgrowth under Insufficient Nerve Growth Factor Condition in PC12 Cells through ERK1/2 Pathway and SIRT1 Modulation

Figure 7

Effects of sesamin on SIRT1 expression and intracellular localization. (a) PC12 cells were treated with 20 ng/ml of NGF alone or combined with sesamin 10 μM for 72 h and then the SIRT1 localization was observed by immunofluorescence staining. Next, PC12 cells were treated with 20 ng/ml of NGF alone, 40 ng/ml of NGF alone, and combination treatment with various sesamin concentrations (1, 5, and 10 μM). (b) After 24 h, the mRNA level of SIRT1 was determined by real-time PCR. (c) At 72 h, the SIRT1 protein was measured by western blot analysis and the band intensity was quantified by TotalLab TL120. Data represent the means ± SD of three independent experiments. compared with the control group; and compared with the NGF alone-treated group. SE: sesamin; NGF: nerve growth factor.

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