Adlay Seed (Coix lacryma-jobi L.) Extracts Exhibit a Prophylactic Effect on Diet-Induced Metabolic Dysfunction and Nonalcoholic Fatty Liver Disease in Mice

Figure 5

The mechanism of ASE and ASW on improving hepatic steatosis in OA-treated HuS-E/2 cells. (a and b) The protein factors related to hepatic de novo lipogenesis and fatty acid β-oxidation pathway were analyzed by western blotting. The protein expression of phosphorylation of AMPK at Thr172 and ACC at Ser79, total AMPK, and ACC under the condition of ASE treatment are shown in panel (a). Those protein expressions under the condition of ASW treatment are shown in panel (b). Tubulin served as a loading control. Quantitative analysis with Multi Gauge V3.0 is shown. (c–f) The gene expression related to hepatic de novo lipogenesis and fatty acid β-oxidation pathway is analyzed by RT-qPCR. The expressions of fatty acid synthesis-related genes: (c) FAS and (d) SREBP-1c. The expression of fatty acid β-oxidation-related genes: (e) PPARα and (f) PPARδ. Experiments are performed in triplicate, and data are shown as means ± SEM. OA vs. ASE or ASW, ; ; in increased level. OA vs. ASE or ASW, ; in decreased level.

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