Effect of Coptis chinensis on Biofilm Formation and Antibiotic Susceptibility in Mycobacterium abscessus

Figure 2

Comparative effects of indicated concentration of C. chinensis and berberine-HCl on the biofilm formation of M. abscessus. M. abscessus was grown in 7H9 broth (control) and under the concentrations of ¼ × MIC, ½ × MIC, MIC, and 2 × MIC of C. chinensis (a) and berberine-HCl (b) at 30°C for 14 days. M. abscessus solution was incubated at 30°C for 14 days (i). After 14 days, nonadherent M. abscessus was removed (ii). The adherent M. abscessus was stained with 3 mL of 0.1% safranin solution at room temperature for 30 min (iii). The safranin stained biofilm was solubilized in acetic acid and then was removed to 96-well PVC plates for measuring the differences in optical density 492 (λ = 492 nm) (iv). The photo represented biofilm solutions of control and indicated concentrations of C. chinensis and berberine-HCl. Mean values of three independent experiments and standard deviations were shown. The Mann–Whitney Rank Sum Test was used to calculate statistical significance. An asterisk indicates a significant difference between the control group and indicated concentration groups. Statistically significant inhibition on the biofilm formation of M. abscessus was observed for 1/4 × MIC, 1/2 × MIC, MIC, and 2 × MIC concentrations of C. chinensis and 1/2 × MIC, MIC, and 2 × MIC concentrations of berberine-HCl. CTL denotes control. MIC denotes minimal inhibitory concentration.

(a)

(b)