HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus

Table 1

Information for quantitative analysis from the monitoring ion.

No.	Component	Rt (min)	Ionization mode	m/z	Herb	Compound class
1	Catalpol	5.06	[M + HCOO]^−	407.10	RR^a	Glycosides
2	Aucubin	8.40	[M + HCOO]^−	391.10	RR	Glycosides
3	Gallic acid	8.58	[M − H]^−	169.10	RR, RRER^b	Organic acid
4	Rhmannioside D	9.67	[M + HCOO]^−	731.10	RR	Glycosides
5	Leonuride	14.18	[M + HCOO]^−	393.10	RR	Glycosides
6	Hydroxysafflor yellow A	18.12	[M − H]^−	611.10	CF^c	Flavonoids
7	Amygdalin	18.78	[M + HCOO]^−	502.10	PS^d	Glycosides
8	Echinacoside	19.87	[M − H]^−	785.15	RR	Glycosides
9	Calycosin-7-O-β-D-glucoside	22.24	[M + H]^+	447.05	AR^e	Flavonoids
10	Acteoside	22.53	[M − H]^−	623.15	RR	Glycosides
11	Ononin	26.59	[M + HCOO]^−	475.05	AR	Glycosides
12	Calycosin	29.34	[M + H]^+	285.10	AR	Flavonoids
13	Astragaloside IV	31.77	[M + HCOO]^−	829.35	AR	Saponins
14	Astragaloside III	32.35	[M + HCOO]^−	829.35	AR	Saponins
15	Ophiopojaponin C	33.19	[M + HCOO]^−	931.35	OR^f	Saponins
16	Astragaloside II	33.93	[M + HCOO]^−	871.40	AR	Saponins
17	Formononetin	34.99	[M + H]^+	269.10	AR	Flavonoids
18	Isoastragaloside II	35.25	[M + HCOO]^−	871.40	AR	Saponins
19	Rhein	37.61	[M − H]^−	283.05	RRER	Anthraquinones
20	Astragaloside I	38.12	[M + HCOO]^−	913.35	AR	Saponins
21	Isoastragaloside I	39.33	[M + HCOO]^−	913.35	AR	Saponins
22	Emodin	43.27	[M − H]^−	269.05	RRER	Anthraquinones

^aRR: Rehmanniae Radix; ^bRRER: Rhei Radix et Rhizoma; ^cCF: Carthami Flos; ^dPS: Persicae Semen; ^eAR: Astragali Radix; ^fOR: Ophiopogonis Radix.