A Metabolomic Investigation of Eugenol on Colorectal Cancer Cell Line HT-29 by Modifying the Expression of APC, p53, and KRAS Genes

Table 1

List of primers, nucleotide sequence, and melting points of primers.

Primer name	Forward 5′ to 3′	Reverse 5′ to 3′	Tm°C
			Forward	Reverse
P53	GCCCAACAACACCAGCTCCT	CCTGGGCATCCTTGAGTTCC	60.88	60.76
KRAS	CTATTCGCAGCTCACACAGTTTAC	TTCTTAATTTGGTCTGCGGC	60.11	60.18
APC	GACTGGTATTACGCTCAACTTCA	CAATTGCCTTCTGGTCATATCTG	60.05	60.08
GAPDH	AGGGCTGCTTTTAACTCTGG	CCCCACTTGATTTTGGAGGG	59.04	60.27