Evidence-Based Complementary and Alternative Medicine

Research Article

A Metabolomic Investigation of Eugenol on Colorectal Cancer Cell Line HT-29 by Modifying the Expression of APC, p53, and KRAS Genes

Table 3

Results of the integrated analysis of metabolic cycles and genes from the MetaboAnalyst database and their relationship with potential genetic changes in colorectal cancer.


Metabolic pathway	Participating metabolites	Levels of metabolites	Total no. of compounds in the pathway	Hits actual number of matched numbers	Pathway impact value	Raw	Change in gene expression
							APC 2-fold>+	P53 3-fold>+	KRAS 0.3<−

Aminoacyl-tRNA biosynthesis	L-glutamate L-valine L-isoleucine L-lysine L-proline L-tyrosine L-tryptophan	↓	74	7	0.09589	1.4684E − 6	✓	✓	✓
		↓
		↓
		↓
		↓
		↓
		↓
		↓
Valine, leucine, and isoleucine biosynthesis	L-valine L-isoleucine	↓	12	2	0.18182	0.0044219	—	—	✓
		↓
Biotin metabolism	L-lysine Biotin	↓	21	2	0.3	0.013428	—	✓	✓

Steroid biosynthesis	Lathosterol Cholesterol Cholesterol ester	↓	82	3	0.16049	0.031442	—	✓	✓
		↓
		↓
Pantothenate and CoA biosynthesis	L-valine Pantothenate	↓	34	2	0.12121	0.033545	✓	✓	—

Glycerolipid metabolism	Phosphatidate Triacylglycerol	↓	35	2	0.20588	0.035395	—	✓	—
		↓
Galactose metabolism	alpha-D-Glucose D-glucose		51	2	0.22	0.06987	✓	✓	✓

D-glutamine and D-glutamate metabolism	L-glutamate		10	1	0.33333	0.082955	—	✓	✓

shows increase and

shows decrease of the metabolite level. ✓ shows involvement of genes in the cycle.