Luteolin Improves Cyclophosphamide-Induced Cystitis through TXNIP/NLRP3 and NF-κB Pathways

Figure 3

LUT reduces apoptosis in CYP-induced cystitis. (a) TUNEL staining of bladder slices in each group. Apoptotic cells were stained dark brown and nonapoptotic cells were stained blue. (b) Statistics of apoptotic index from TUNEL staining in each group (n = 5). LUT reduced the number of TUNEL staining positive cells in the bladder. (c) The expression of Bax, Bcl-2, caspase-3, and caspase-8 in the bladder of each group was detected by western blot. LUT treatment ameliorated the disorder of apoptosis-related proteins in CYP-induced cystitis. The expression analysis of Bax (d), Bcl-2 (e), caspase-3 (f), and caspase-8 (g) proteins in the bladder of each group (n = 6). ^∗, , and ; NS: not significant.

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