Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

Table 2

The effect of controls, hydroethanolic root extract, and solvent fractions of Cyphostemma adenocaule on delayed-type hypersensitivity response and organ index.

Treatment group	Dose (mg/kg)	DTH (mean ± SEM)	Swelling (%)	Organ index (mg/g)
				Spleen	Liver
VH	—	0.065 ± 0.013	—	4.594 ± 0.123	44.35 ± 0.862
LEV	50	0.112 ± 0.013^c1	↑41.96	5.016 ± 0.200^c3	52.000 ± 1.718^a3c3
CYP	30	0.055 ± 0.015	↓18.18	3.859 ± 0.094	39.981 ± 0.564
HEE	100	0.091 ± 0.003	↑28.57	4.778 ± 0.107^c3	48.000 ± 0.807^c3
	200	0.113 ± 0.011	↑42.48	5.094 ± 0.114^c3	48.330 ± 1.100^c3
	400	0.123 ± 0.010^c1	↑47.15	5.359 ± 0.174^a2c3g1	52.381 ± 1.720^a3c3
AF	100	0.081 ± 0.007	↑19.75	4.716 ± 0.128^c3	47.592 ± 1.122^c3
	200	0.092 ± 0.005	↑29.35	4.861 ± 0.095^c3	47.800 ± 1.098^c3
	400	0.109 ± 0.011^a1c3	↑40.37	4.967 ± 0.131^c3	47.912 ± 0.940^c3
BF	100	0.086 ± 0.013	↑24.42	4.768 ± 099^c3	47.660 ± 1.202^c3
	200	0.100 ± 0.007	↑35.00	4.839 ± 0.095^c3	48.040 ± 1.006^c3
	400	0.113 ± 0.012^c1	↑42.48	5.346 ± 0.164^a2c3	51.815 ± 1.131^a3c3

Values are expressed as mean ± SEM, n = 6. Abbreviations: DTH = delayed-type hypersensitivity, VH = vehicle (2% gum acacia), LEV = levamisole, CYP = cyclophosphamide, HEE = hydroethanolic extract, AF = aqueous fraction, BF = n-butanol fraction, ↑ = increase in paw volume, ↓ = decrease in paw volume, a = compared to vehicle, c = compared to cyclophosphamide, g = compared to 100 mg/kg aqueous fraction, .