Ferulic Acid Alleviates Oxidative Stress-Induced Cardiomyocyte Injury by the Regulation of miR-499-5p/p21 Signal Cascade

Figure 1

Ferulic acid protects cardiomyocytes from H₂O₂-induced cell injury by attenuating oxidative stress. (a) H9c2 cells were incubated with 0–100 μM ferulic acid for 24 hrs, and the cell viability was analyzed by MTT assay (n = 4). (b) H9c2 cells were treated with 0–100 μM ferulic acid and 200 μM H₂O₂ for 24 hrs, and the cell viability was determined by the MTT assay (n = 5). (c) The viability of the cells in the control (Con), 200 μM H₂O₂ (H₂O₂), 200 μM H₂O₂ + 50 μM ferulic acid (H₂O₂ + FA), and 50 μM ferulic acid (FA) groups (n = 4). (d), (e) Reactive oxygen species (ROS) analysis by DHE staining in the Con-, H₂O₂-, H₂O₂ + FA-, and FA-treated cells (n = 4). ^∗ vs. control, ^∗∗ vs. control, ^∗∗∗ vs. control, ^# vs. H₂O₂, and ^## vs. H₂O₂. FA indicates ferulic acid.

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