Ferulic Acid Alleviates Oxidative Stress-Induced Cardiomyocyte Injury by the Regulation of miR-499-5p/p21 Signal Cascade

Figure 2

Ferulic acid attenuates H₂O₂-induced cell apoptosis in vitro. H9c2 cells were pretreated with 50 μM ferulic acid for 4 hrs and then incubated with 200 μM H₂O₂ for 24 hrs. (a) A representative image of DNA fragments analysis (DNA laddering) of Con-, H₂O₂-, H₂O₂ + FA-, and FA-treated cells. (b) Cell apoptosis was analyzed by Hoechst 33342 staining in the Con, H₂O₂, H₂O₂ + FA, and FA groups (n = 4). (c). (d) Representative TUNEL staining photos and the averaged data from the Con-, H₂O₂-, H₂O₂ + FA-, and FA-treated H9c2 cells (n = 4). (e), (f) Representative DHE staining photos and the averaged data from the Con, 10 μM isoproterenol (ISO), 10 μM isoproterenol + 50 μM ferulic acid (ISO + FA), and FA groups (n = 4). (g). (h) Representative TUNEL staining photos and the averaged data from the Con, ISO, ISO + FA, and FA groups (n = 4). ^∗, ^∗∗, and ^∗∗∗.

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