Ferulic Acid Alleviates Oxidative Stress-Induced Cardiomyocyte Injury by the Regulation of miR-499-5p/p21 Signal Cascade

Figure 4

MiR-499-5p is a key regulator of the protective effect of ferulic acid in oxidative stress-induced cardiomyocyte injury. (a), (b) Cell apoptosis was analyzed by TUNEL staining in the Con, H₂O₂, and H₂O₂ + miRNA negative control (H₂O₂ + Nc), H₂O₂ + miR-499-5p mimics (H₂O₂ + miR-499-5p), H₂O₂ + ferulic acid + miRNA inhibitor negative control (H₂O₂ + FA + Nci), and H₂O₂ + ferulic acid + miR-499-5p inhibitor (H₂O₂ + FA + miR-499-5pi) groups (n = 4). (c) Cell viability was analyzed in the Con, H₂O₂, H₂O₂ + Nc, H₂O₂ + miR-499-5p, H₂O₂ + FA + Nci, and H₂O₂ + FA + miR-499-5pi groups by MTT assay (n = 4). (d) Cell apoptosis was detected by DNA laddering of Con, H₂O₂, H₂O₂ + Nc, H₂O₂ + miR-499-5p, H₂O₂ + FA + Nci, and H₂O₂ + FA + miR-499-5pi group cells. (e), (f), (g), (h), and (i) Western blot and the average protein levels data of p21, phosphorylatedp38 (p-p38), p38, Bcl-2, Bax, and c-caspase-3 in the Con, H₂O₂, H₂O₂ + FA + Nci, and H₂O₂ + FA + miR-499-5pi groups. GAPDH was used as the protein loading control. Cropped blots, n = 4. ^∗, ^∗∗, and ^∗∗∗.

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