Ferulic Acid Alleviates Oxidative Stress-Induced Cardiomyocyte Injury by the Regulation of miR-499-5p/p21 Signal Cascade

Table 1

Sequences for cell transfection and primers for RT–PCR.

Group name	Sequences
miR-499-5p mimic	Sense: 5′-UUAAGACUUGCAGUGAUGUUU-3′
	Antisense: 5′-AAACAUCACUGCAAGUCUUAA-3′
Negative control (nc)	Sense: 5′-UCACAACCUCCUAGAAAGAGUAGA-3′
	Antisense: 5′-UCUACUCUUUCUAGGAGGUUGUGA-3′
miR-499-5p inhibitor	5′-AAACAUCACUGCAAGUCUUAA-3′
Negative control of miRNA inhibitor (nci)	5′-CAGUACUUUUGUGUAGUACAA-3′
Rattus-miR-499-5p	RT primer: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAACATC-3′ PCR forward primer: 5′-CGTCCGATTAAGACTTGCAGT-3′
U6	RT primer: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATG-3′ PCR forward primer: 5′-GCGCGTCGTGAAGCGTTC-3′
Universal PCR reverse primer for miRNA and U6	5′-GTGCAGGGTCCGAGGT-3’
Mus-p21 PCR primers	5’-GTACTTCCTCTGCCCTGCTG-3′ 5′- AGAGTGCAAGACAGCGACAA-3’
Mus-col1a1 PCR primers	5’-GAGCGGAGAGTACTGGATCG-3′ 5′-GTTCGGGCTGATGTACCAGT-3′