Sex-Biased Gene Expression of Mesobuthus martensii Collected from Gansu Province, China, Reveals Their Different Therapeutic Potentials

Figure 6

Sequence alignment, 3D modeling and phylogenetic analysis of a disintegrin and metalloproteinase. (a) A putative sequence Trinity_DN16345_c6_g12 was aligned with a model Adam (PDB ID: 6BE6). At the bottom of columns, asterisk () shows conserved positions, colons (:) shows conserved substitutions, and points (.) shows nonconserved substitutions. Grey lines, green bends, blue-banded arrowheads, and red solenoids represent coils, turns, sheets, and helices respectively. Different fragments are framed by red lines. (b) 3D modeling was simulated by the template ADAM (PDB ID: 6BE6) in SWISS-MODEL and viewed in Discovery Studio 2016. The colors grey, green, blue, and red represent coils, turns, sheets, and helices, respectively. Different structures are indicated by red arrows. (c) Phylogenetic tree was constructed with sequence Trinity_DN16345_c6_g12, which is a putative ADAM and 10 other sequences from different species in MEGA 7, by the Neighbor-Joining method. The putative ADAM is marked in a red dot, arthropoda are marked in light blue dots, and Angiospermae are marked in a yellow dot.

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