Sex-Biased Gene Expression of Mesobuthus martensii Collected from Gansu Province, China, Reveals Their Different Therapeutic Potentials

Figure 7

Sequence alignment, 3D modeling, and phylogenetic analysis of cathepsin L. (a) A putative sequenceTrinity_DN20786_c0_g1 was aligned with a model CTSL (PDB ID: 6JD8). At the bottom of columns, asterisk () shows conserved positions, colons (:) shows conserved substitutions, and points (.) shows nonconserved substitutions. Grey lines, green bends, blue-banded arrowheads, and red solenoids represent coils, turns, sheets and helices respectively. Different fragments are framed by red lines. (b) 3D modeling was simulated by the template CTSL (PDB ID: 6JD8) in SWISS-MODEL and viewed in Discovery Studio 2016. The grey, green, blue, and red represent coils, turns, sheets and helices, respectively. Different structures are indicated with red arrows. (c) Phylogenetic tree was constructed with sequence Trinity_DN20786_c0_g1, which is a putative CTSL and 10 other sequences from different species, in MEGA 7, by the Neighbor-Joining method. The putative CTSL is marked in a red dot, arthropoda in light blue dots, priapulida in a purple dot, ollusca in a green dot, and brachiopoda in a pink dot.

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