Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1

Figure 4

(a) Western blot detection of relative expression of NRG1 and ErbB4 in hippocampal tissues (n = 4, one-way ANOVA, ). Data are presented as means ± SD, vs. Con group; vs. Meth group. (b) Schematic protocol of transfection (created with BioRender.com). H9c2 cell transfection efficiency of overexpressed miR-183-5p (miR-183-5p-mimic). Data are presented as means ± SD, vs. NC-mimic group. (c) Relative expression of NRG1 in HT22 cells detected via western blot (n = 4, one-way ANOVA, ). Data are presented as means ± SD, vs. Control, vs. Meth group. Mimic + Meth: H9c2-transfected miR-183-5p-mimic with Rhy + Meth coincubation; NC + Meth: H9c2-transfected miR-NC-mimic with Rhy + Meth coincubation; Rhy + Meth: H9c2 with Rhy + Meth coincubation group. (d) Dual-luciferase assay of miR-183-5p targeting effects on NRG1 3′-UTR (n = 3, t-test, ). Data are presented as means ± SD, .

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