Anti-Inflammatory Effects of Lagerstroemia ovalifolia Teijsm. & Binn. in TNFα/IFNγ-Stimulated Keratinocytes

Figure 5

LOC reduces NF-κB activation and translocation. (a, c) HaCaT cells were pretreated with LOC for 1 h and then stimulated with TNFα/IFNγ for 2 h. The phosphorylation levels of the indicated proteins were determined in whole-cell lysates by western blotting. (b) The translocation of NF-κB p65 was confirmed through immunocytochemistry as described in the Section 2. (d) Luciferase activity was measured in the presence of LOC. LOC (2.5, 5, 10, and 20 µg/ml) was processed, and after 1 h, TNFα/IFNγ (10 ng/ml) was added. After 16 h, luciferase activity was measured. The data are presented as the mean ± SEM of three samples. Statistically significant differences ( and ) between the treatment groups are indicated by different letters.

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