Inhibitory Effect of Gualou Guizhi Decoction on Microglial Inflammation and Neuron Injury by Promoting Anti-Inflammation via Targeting mmu-miR-155

Figure 3

GLGZD prominently restricts the level of mmu-miR-155 and promotes the expression of its targeting meditators. (a) The mRNA levels of mmu-miR-155 and SOCS1, SMAD2, SHIP1, and TAB2 measured by RT-PCR and quantitative assessment performed by normalizing to the level of internal control GAPDH. (b) The protein expression level of SOCS1, SMAD2, SHIP1, and TAB2 assessed by Western blotting assay and normalized to the level of internal control β-actin. β-Actin was used for normalization, and the intensity of bands was quantified by densitometric analysis. All values represent mean ± SD of three independent experiments. , vs. control and , vs. OGD condition.

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