Intraspecific DNA Barcoding and Variation Analysis for Citri Reticulatae Pericarpium of Citrus reticulata “Chachi”

Table 2

Primer sequence and PCR system of DNA barcodings.

Barcoding	Direction	Primer sequence (5′⟶3′)	PCR system	TM (°C)
ITS2	F	ATGCGATACTTGGTGTGAAT	98°C 2 min; 98°C 10 s, TM °C 10 s, 72°C 10 s; 72°C 5 min	58
	R	GACGCTTCTCCAGACTACAAT
rbcl	F	ATGTCACCACAAACAGAAAC		58
	R	TCGCATGTACCTGCAGTAGC
matK	F	AGAGGTATTTGCTGCTGTGGTG		58
	R	GGAAAGAGTAAAGCAAGAACGTGT
psbA-trnH	F	AGGTATCTGGTTCACTGCTTTAGGT		59
	R	GCCTTGATCCACTTGGCTACAT

TM, The melting temperature of DNA.