Antihypertensive Effects of the Methanol Extract and the Ethyl Acetate Fraction from Crinum zeylanicum (Amaryllidaceae) Leaves in L-NAME-Treated Rat
Table 2
Effect of chronic administration of captopril, methanol extract, and ethyl acetate fraction obtained from leaves of Crinum zeylanicum on diuresis, glomerular filtration rate, plasma and urine nitric oxide, proteins, and creatinine in L-NAME treated rats.
Parameters
Treatments
Naive
L-NAME
L-NAME + capto20
L-NAME + LME 30
L-NAME + LME 60
L-NAME + LME 120
L-NAME + LEAF 30
L-NAME + LEAF 60
L-NAME + LEAF 120
Diuresis (ml/kg/h)
1.07 ± 0.11
1.26 ± 0.08
1.43 ± 0.23
1.70 ± 0.17
1.71 ± 0.23
1.77 ± 0.30
0.810 ± 0.186
0.68 ± 0.09
1.59 ± 0.18
NO (µg/µl)
Plasma
4.35 ± 0.70
4.41 ± 0.31
5.80 ± 0.37
3.20 ± 0.39
5.06 ± 0.37
4.58 ± 0.31
5.70 ± 0.33
4.78 ± 0.26
5.35 ± 0.53
Urine
1.99 ± 0.27$$$
5.05 ± 0.49
2.57 ± 0.92$$
2.31 ± 0.35$$$
0.60 ± 0.05$$$
0.54 ± 0.05$$$
0.64 ± 0.06$$$
0.53 ± 0.10$$$
0.42 ± 0.04$$$
Proteins (µg/ml)
Plasma
6.01 ± 0.63$
9.54 ± 0.44
7.25 ± 0.40
5.62 ± 1.01$$
4.05 ± 0.48$$$
4.33 ± 0.98$$$
6.73 ± 1.03
7.52 ± 1.21
3.40 ± 1.13$$$
Urine
9.59 ± 0.35
9.85 ± 1.11
5.92 ± 0.251$
5.30 ± 0.77$$
6.33 ± 0.98$
8.61 ± 0.89
5.90 ± 0.88$$
6.46 ± 0.65$
5.26 ± 0.58$$$
Creatinine (mg/ml)
Plasma
3.66 ± 0.48
2.85 ± 0.55
2.60 ± 0.26
2.47 ± 0.20
2.42 ± 0.41
2.38 ± 0.33
2.66 ± 0.34
2.88 ± 0.49
4.33 ± 0.52
Urine
3.47 ± 0.44$$$
1.02 ± 0.04
3.20 ± 0.55$$$
1.14 ± 0.16
1.49 ± 0.14
6.06 ± 0.37$$$
1.16 ± 0.19
5.11 ± 0.50$$$
2.19 ± 0.36$$$
GFR (µL/min)
0.37 ± 0.06$
0.10 ± 0.01
0.35 ± 0.05$
0.20 ± 0.04
0.24 ± 0.03
1.01 ± 0.16$$$
0.10 ± 0.02
0.31 ± 0.07
0.23 ± 0.05
L-NAME: Nω-nitro-L-arginine methyl-ester, LME: leaf methanol extract, LEAF: leaf ethyl acetate fraction, capto20: captopril, and naive: normal untreated animals. The number associated with the administered substances indicates the oral dose in mg/kg/day. 6 ≤ n ≤ 8. Analysis was done with ANOVA one-way followed by Tukey posttest. ,, and : significant difference as compared to L-NAME treated group.
