Phytochemical Analysis and Investigation of the Antimicrobial and Cytotoxic Activities of Croton dichogamus Pax Crude Root Extracts

Table 2

Antimicrobial activity of C. dichogamus root extracts on various concentrations using the agar well diffusion technique.

Microorganisms	Concentration	Zone of inhibition (MM)
	mg/ml	Acetone extract	Hydroethanolic extract	Aqueous extract	Positive control	Negative control
					Cephalexin
Bacillus cereus	250	17.33 ± 0.33	14.33 ± 0.33	12.83 ± 0.93	29.67 ± 2.48	0.00 ± 0.00
	125	16.33 ± 0.88	12.33 ± 0.33	10.33 ± 0.33
	62.5	13.67 ± 0.88	9.5 ± 1.04	8.50 ± 0.5
	31.25	5.67 ± 2.85	5.33 ± 2.68	2.50 ± 2.50
	15.63	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	7.81	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Staphylococcus aureus	250	12.33 ± 0.88	11.5 ± 0.29	10.67 ± 0.44	28.67 ± 0.58	0.00 ± 0.00
	125	11.33 ± 0.33	10.33 ± 0.33	9.33 ± 0.60
	62.5	9.67 ± 0.33	8.17 ± 0.44	8.00 ± 0.00
	31.25	2.67 ± 2.67	0.00 ± 0.00	2.50 ± 2.50
	15.63	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	7.81	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Pseudomonas aeruginosa	250	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	27.33 ± 0.58	0.00 ± 0.00
Escherichia coli	250	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	28.66 ± 4.16	0.00 ± 0.00
					Fluconazole
Candida albicans	250	15.00 ± 0.58	12.00 ± 0.58	9.33 ± 0.33	29.00 ± 2.52	0.00 ± 0.00
	125	13.67 ± 0.33	10.33 ± 0.33	8.83 ± 0.33
	62.5	10.67 ± 0.33	8.33 ± 0.33	5.33 ± 2.67
	31.25	5.33 ± 2.67	5.17 ± 2.59	2.50 ± 2.50
	15.63	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	7.81	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00

Zones of inhibition were expressed as mean ± SEM of the triplicate experiments. 0.00 = no activity.