Highly Expressed CYBRD1 Associated with Glioma Recurrence Regulates the Immune Response of Glioma Cells to Interferon

<div><i>In vitro</i> effects of CYBRD1 on glioma cell phenotype. (a) CYBRD1 overexpression or silencing was achieved in glioma LN229 and T98G cells by transfecting pcDNA3.1/CYBRD1 OE or small interfering RNA targeting CYBRD1 (si-CYBRD1); pcDNA3.1 or si-NC was transfected as a negative control. The transfection efficiency was verified by RT-qPCR. Then, LN229 and T98G cells were transfected with CYBRD1 OE or si-CYBRD1 and examined for cell viability by CCK-8 assay (b); cell migration by wound healing assay (c); cell invasion by transwell assay (d). <span class="nowrap"><svg height="13.532pt" id="M7" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 56.1861 13.532" width="56.1861pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 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Evidence-Based Complementary and Alternative Medicine

fig3

Figure 3

Figure 3: Highly Expressed CYBRD1 Associated with Glioma Recurrence Regulates the Immune Response of Glioma Cells to Interferon 