Quercetin Reduces Oxidative Stress and Apoptosis by Inhibiting HMGB1 and Its Translocation, Thereby Alleviating Liver Injury in ACLF Rats

Figure 1

Establishment of ACLF rat model and experimental intervention. The rats were injected with HSA to induce immune hepatic fibrosis. At first stage, rats were sensitized by subcutaneous injection of HSA solution (0.5 ml, HSA 4 mg) for a total of 4 injections (days 0, 14, 24, and 34). Subsequently, tail vein injection was performed twice a week for 6 weeks (0.5 ml, gradually increased the HSA dose, 2.5 mg⟶3 mg⟶3.5 mg⟶4 mg⟶4.5 mg, and then maintained at 4.5 mg), and the normal group was injected with the same amount of normal saline. And then, intraperitoneal injection of 400 mg/kg D-GaLN and 100 μg/kg LPS caused acute liver injury to establish the ACLF model. Finally, the rats were randomly divided into 5 intervention groups: receiving Que and/or glycyrrhizin, or vehicle treatment for 7 consecutive days. The normal control group underwent the same procedures without therapeutic intervention.