[Retracted] LncRNA-URHC Functions as ceRNA to Regulate DNAJB9 Expression by Competitively Binding to miR-5007-3p in Hepatocellular Carcinoma

Figure 3

URHC was a target of miR-5007-3p. (a) Relative expression of miR-5007-3p in HCC cell lines and normal hepatic cells was detected by qRT-PCR. (b) Relative expression of miR-5007-3p in HCC tissues and adjacent tissues was detected by qRT-PCR. (c) RT-qPCR analysis of URHC-regulated miR-5007-3p expression in 7721, 7402, 7404, and Hep3B cells. (d) The expression level of miR-5007-3p in tumors was detected. (e) FISH was performed to analyze the colocalization of URHC and miR-5007-3p in HCC cell lines and tissues. Magnification: ×400. (f) The predicted miR-5007-3p binding sites in URHC-WT and the designed mutant sequence (URHC-MUT) were indicated. (g) Luciferase reporter assay of 293T cells cotransfected with URHC-WT or URHC-MUT and miR-5007-3p NC or miR-5007-3p. The bars represent means ± SD of three independent experiments. , , and compared with the control group.

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