[Retracted] LncRNA-URHC Functions as ceRNA to Regulate DNAJB9 Expression by Competitively Binding to miR-5007-3p in Hepatocellular Carcinoma

Figure 5

URHC regulated the expression of DNAJB9, an endogenous miR-5007-3p target. (a) Relative expression of DNAJB9 in HCC tissues and adjacent tissues was detected by qRT-PCR. (b) RT-qPCR analysis of URHC-regulated DNAJB9 expression in 7721, 7402, 7404, and Hep3B cells. (c, d) The mRNA and protein levels of DNAJB9 in tumors were assessed. (e) The positive correlation between URHC and miR-5007-3p expression and the negative correlation between miR-5007-3p and DNAJB9 expression in HCC tissues are shown in scatter diagrams. (f) The predicted binding sites of miR-5007-3p in the DNAJB9 sequence are shown. (g) Luciferase reporter assay of 293T cells cotransfected with DNAJB9-WT or DNAJB9-MUT and miR-5007-3p NC or miR-5007-3p. (h, i) The levels of DNAJB9 in HCC cells transfected with miR-5007-3p inhibitor/mimic or siRNA-URHC/over-URHC were analyzed by RT-qPCR and western blot. , , and .

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