Simultaneous Determination of Ten Bioactive Components from Shenling Baizhu San in Rat Plasma by UHPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Two Models of Ulcerative Colitis Rats

Table 3

Extract recovery, matrix effect, precision, and accuracy data of the analytes in rat plasma (n = 5).

Analyte	Concentration (ng·mL^−1)	Absolute recovery (%)	Absolute matrix effect (%)	Relative matrix effect (%)	Precision% (RSD)		Accuracy% (Mean ± SD)
					Intraday	Interday	Intraday	Interday
PAN	0.80	93.68 ± 8.33	52.84 ± 2.44	6.59	12.14	12.14	82.34 ± 5.42	103.54 ± 13.47
	99.38	93.06 ± 8.24	56.56 ± 2.35	7.81	7.60	8.20	101.60 ± 8.22	99.97 ± 6.012
	397.50	92.74 ± 4.43	46.34 ± 9.01	9.86	6.59	6.47	104.46 ± 3.79	99.99 ± 17.16
Rg1	1.14	66.92 ± 7.47	102.59 ± 1.56	11.58	10.41	9.95	98.3 ± 8.08	99.75 ± 2.79
	142.13	65.06 ± 5.40	93.92 ± 5.68	13.05	13.60	10.41	114.75 ± 1.30	90.02 ± 2.20
	568.50	53.71 ± 1.14	46.65 ± 12.63	11.49	11.91	13.60	91.57 ± 2.80	92.01 ± 3.50
ATA-I	0.80	56.89 ± 7.00	75.07 ± 13.32	8.17	4.47	1.89	119.38 ± 7.10	93.75 ± 1.08
	100.13	65.26 ± 1.00	70.17 ± 4.24	7.82	1.85	3.78	90.83 ± 4.47	100.02 ± 2.23
	400.50	77.85 ± 1.44	67.40 ± 3.57	5.88	13.30	12.47	102.29 ± 11.23	99.03 ± 5.96
ATA-III	0.98	66.92 ± 7.47	48.99 ± 3.94	5.54	6.69	8.09	119.25 ± 10.37	101.10 ± 6.55
	122.63	65.06 ± 5.4	93.56 ± 4.43	9.49	8.30	5.90	96.99 ± 10.89	93.58 ± 12.93
	490.50	98.81 ± 5.38	82.58 ± 9.47	11.67	11.17	10.84	100.61 ± 6.42	95.84 ± 7.01
PA	0.69	79.86 ± 6.63	54.98 ± 12.72	10.73	1.89	9.93	91.11 ± 10	103.41 ± 3.17
	62.25	85.95 ± 12.08	74.95 ± 4.71	10.27	13.89	14.55	98.54 ± 3.25	101.02 ± 2.32
	345.00	99.46 ± 1.89	71.18 ± 9.29	5.84	8.30	9.48	103.59 ± 2.21	94.22 ± 4.09
NEF	0.31	55.13 ± 4.65	41.51 ± 3.49	9.58	6.24	6.10	102.55 ± 2.05	110.41 ± 2.62
	69.75	53.67 ± 1.46	34.17 ± 1.85	10.97	4.77	3.57	109.22 ± 2.13	98.37 ± 4.14
	279.00	78.85 ± 2.97	74.39 ± 1.43	9.71	7.42	10.50	95.57 ± 2.48	94.22 ± 3.09
NUC	0.74	65.79 ± 3.49	58.7 ± 7.32	7.94	11.16	10.13	111.07 ± 1.61	99.77 ± 3.38
	69.75	57.76 ± 10.04	73.32 ± 3.94	9.38	10.11	9.08	111.73 ± 5.80	101.44 ± 5.30
	225.00	65.79 ± 6.17	56.92 ± 5.47	13.86	5.30	10.49	96.17 ± 5.32	99.25 ± 9.69
DG	0.89	73.97 ± 2.81	77.47 ± 8.16	11.71	14.55	7.23	106.74 ± 1.36	102.68 ± 3.72
	111.75	60.05 ± 8.30	127.46 ± 9.34	5.81	8.86	10.44	100.01 ± 1.5	100.89 ± 3.7
	447.00	55.79 ± 1.04	75.49 ± 9.74	8.43	13.8	12.31	104.28 ± 1.78	94.98 ± 1.87
PD	0.77	94.58 ± 5.68	58.48 ± 11.23	10.73	6.01	6.84	99.95 ± 6.42	107.93 ± 3.31
	95.63	84.78 ± 12.69	135.38 ± 15.43	5.84	12.69	12.67	100.20 ± 10.89	108.66 ± 1.76
	382.50	61.01 ± 5.75	115.65 ± 5.36	10.27	12.15	12.91	109.25 ± 10.37	105.23 ± 1.01
ISL	0.71	68.86 ± 8.93	54.78 ± 5.27	12.50	1.61	7.064	96.40 ± 7.149	100.76 ± 9.77
	88.86	65.71 ± 5.00	89.26 ± 6.30	13.44	7.60	10.32	98.27 ± 5.47	95.410 ± 3.09
	355.50	64.84 ± 4.88	85.51 ± 8.51	14.39	12.96	12.0	100.72 ± 3.7	93.01 ± 12.45
IS	104	94.51 ± 4.29	99.84 ± 7.96	—	—	—	—	—