Research Article
Wang-Bi Tablet Ameliorates DMM-Induced Knee Osteoarthritis through Suppressing the Activation of p38-MAPK and NF-κB Signaling Pathways in Mice
Figure 6
Effect of WBT on the phosphorylation of NF-κB p65, p38 signaling pathways, and inflammation. Chondrocytes were treated with normal serum medium, WBT serum medium, WBL (low dose of WBT) serum medium, WBM (medium dose of WBT) serum medium, and WBH (high dose of WBT), followed by IL-1β stimulation. (a) Equal amount of proteins from the chondrocytes in each group were merged, and samples (30 μg) were subjected to immunoblotting with indicated antibodies. (b) Densitometry quantification in (a) was analyzed by ImageJ software. Data are means ± SEM. All experiments are repeated for three times at least. (c) The mRNA levels of TNF-α, MMP9, and IL-6 were analyzed by RT-qPCR (a, b, c). , , and vs Nor group; , , and vs Con group.
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