Desensitization of TRPV1 Involved in the Antipruritic Effect of Osthole on Histamine-Induced Scratching Behavior in Mice

Figure 5

Osthole induced calcium influx through TRPV1 in DRG neurons. (a) Representative images in cultured DRG neurons at normal (aA) and after treatment with osthole (30 μM) (aB), pretreatment with AMG9810 (1 μM) (AMG9810 + osthole) (aC). Kcl (aD) was used as the positive control (scale bar: 20 μm). (b) Representative traces showed the neurons activated by osthole were inhibited by pretreatment of TRPV1 antagonist AMG9810. (c) The mean fluorescence intensity of osthole positive neurons were restrained by pretreatment of AMG9810 (0.45 ± 0.06 (n = 262) vs 0.10 ± 0.04 (n = 146), total cells = 1654, ^∗∗P < 0.01, n = 3 animals). (d) The percentage of osthole positive neurons was inhibited by pretreatment of AMG9810 (17.36 ± 3.04% vs 9.49 ± 3.61%, ^∗P < 0.05, total cells = 1654, n = 3 animals).

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